To support the fight against Neglected Tropical Diseases (NTDs), and following the guidelines of the World Health Organization and the Sustainable Development Goals, at Kveloce we have successfully completed the project ‘Feasibility study for the development of a point-of-care diagnostic platform in Sub-Saharan Africa’ (INNTA2/2022/19). This project, led by PhD. Luisa Hueso, is part of our R&D&I line ‘Health and Wellness’. We focus on the co-design and validation of an innovative biosensor platform to rapidly and accurately diagnose different NTDs at the point of care, integrating a social approach that improves access to early diagnosis in vulnerable communities in Sub-Saharan Africa, thus creating the basis for future innovations in the region.
The following are the main results obtained:
- Identification and selection of diagnostic tools for co-design in sub-Saharan Africa: We conducted a comprehensive analysis of the most effective point-of-care diagnostic tools (POCT) for detecting NTDs in resource-limited settings, with a particular focus on sub-Saharan Africa. We evaluated both commercial and in-development diagnostic methods, and selected the most promising ones, such as lateral flow immunoassay (LFA) for antigen detection and recombinase polymerase amplification (RPA) for nucleic acid detection, which allow the diagnosis of several diseases at the same time. In addition, we selected dengue, malaria, chikungunya and Buruli ulcer as priority pathologies for the multiplex biosensor platform. We also identified key biomarkers for each NTD, ensuring rapid and accurate detection that will facilitate access to early diagnosis.
- Prevalence mapping and treatment studies of selected NTDs: We obtained a detailed analysis of the distribution and prevalence of the selected diseases in sub-Saharan Africa and studied their symptomatology, treatments and available vaccines. In addition, we identified cultural and social barriers that could influence the acceptance of diagnostic technologies in these communities. This analysis allowed us to better adapt diagnostic tools to local realities.
- Co-design of POCT prototypes for simultaneous detection: With the information gathered, we co-designed five diagnostic prototypes for simultaneous detection of dengue, malaria and chikungunya, designed to be rapid, accessible and easy to use. We also co-designed an additional prototype for Buruli ulcer detection. During this process, we determined the key reagents and biomolecules to be used in the six multiplexed prototypes and proposed a prototype for laboratory evaluation.
- Proof of concept and technical evaluation of POCT: In collaboration with the biosensor group of the Polytechnic University of Valencia, we performed an accurate assessment of the affinity and selectivity of different antibodies against target antigens. We developed a multiplexed LFA diagnostic assay with colorimetric detection using colloidal gold particles. Although the system requires further optimisation, we obtained very promising results that will lay the foundation for future research. In addition, key strategies for improving and refining the system were identified.
- Study on the impact of infectious diseases on sub-Saharan communities and empowerment mechanisms: We conducted a study to understand the impact of infectious diseases on communities in sub-Saharan Africa and explore ways to empower them in diagnosis and prevention. We developed data collection tools and created an accessible diagnostic awareness guide for diseases such as dengue, malaria and chikungunya. We also established eight strategic partnerships with key actors in the region, broadening the project’s impact and laying the groundwork for future collaborations.
- Dissemination of results: We published a scientific paper on NTD diagnostic tools and presented the prototype at an international conference in poster format. We also created a good practice guide to improve access to diagnostics in underserved communities. In addition, we carried out communication activities such as the creation of audiovisual material and an awareness-raising campaign on NTDs. Finally, we established international collaboration with research centres in Sub-Saharan Africa and Europe, which broadened the scope of the project, enhanced its impact and facilitated participation in international initiatives such as Horizon Europe.
The results have laid the foundation for the development of accessible diagnostic tools and opened the door for future research and international collaborations. Thanks to the progress achieved, we are closer to contributing to the eradication of NTDs and improving the quality of life of millions of people.
We thank all the people and entities that have collaborated with the project and the funding from the Agencia Valenciana de Innovación, the IVACE, and the ERDF, which have made possible this important step towards a more equitable and accessible health system in regions affected by NTDs.
Principal Investigator of NTDs-POCT project